Streptomyces hilarionis sp. nov. and Streptomyces hayashii sp. nov., two new strains associated with potato scab in Brazil

Abstract

Two new actinobacteria, designated strains IBSBF 2807Tand IBSBF 2953T, isolated from scab lesions on potato tubers grown in the southern Brazilian states of Rio Grande do Sul and Santa Catarina, respectively, were characterized and identified through a polyphasic approach. Phylogenetic analyses of 16S rRNA sequences revealed that these two strains belong to the genusStreptomyces. Multilocus sequence analysis using five concatenated genes,atpD,gyrB,recA,rpoBandtrpB, allocated strains IBSBF 2807Tand IBSBF 2953Tin distinct branches ofStreptomycesphytopathogenic strains. PCR-RFLP analysis of theatpDgene also confirmed that these strains differ from the type strains ofStreptomycesassociated with potato scab. The morphological, physiological and biochemical characterization, along with the overall genome-related index properties, indicated that these two strains could be distinguished from their closest phylogenetic relatives and each other. According to the data, IBSBF 2807Tand IBSBF 2953Trepresent two newStreptomycesspecies related to potato scab. The proposed names for these strains areStreptomyces hilarionissp. nov. (IBSBF 2807T=CBMAI 2674T=ICMP 24297T=MUM 22.66T) andStreptomyces hayashiisp. nov (IBSBF 2953T=CBMAI 2675T=ICMP 24301T=MUM 22.68T).