Affiliation:
1. Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Xiangfang District, Harbin 150030, PR China
2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China
Abstract
A novel rhizobacterium, designated strain NEAU-GH312T, with antibacterial activity against
Ralstonia solanacearum
was isolated from rhizosphere soil of rice (Heilongjiang Province, PR China) and characterized with a polyphasic approach. Cells of strain NEAU-GH312T were Gram-stain-negative, aerobic, non-spore-forming, motile with peritrichous flagella and rod-shaped. Colonies were light orange, convex and semi-translucent on Reasoner's 2A (R2A) agar after 2 days of incubation at 28 °C. Growth was observed on R2A agar at 10–40 °C, pH 4.0–8.0 and with 0–5 % (w/v) NaCl. The respiratory quinone was ubiquinone Q-8. The major cellular fatty acids of strain NEAU-GH312T were C16 : 1
ω7c and/or C16 : 1
ω6c, C16 : 0 and C18 : 1
ω7c and/or C18 : 1
ω6c. The main polar lipids were phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. Phylogenetic analyses confirmed the well-supported affiliation of strain NEAU-GH312T within the genus
Massilia
, close to the type strains of
Massilia arvi
THG-RS2OT (98.7 %),
Massilia norwichensis
NS9T (98.7 %) and
Massilia kyonggiensis
TSA1T (98.6 %). Strain NEAU-GH312T had a genome size of 6.68 Mb and an average DNA G+C content of 66.3 mol%. Based on the genotypic, phenotypic and chemotaxonomic data obtained in this study, strain NEAU-GH312T could be classified as representative of a novel species of the genus
Massilia
, for which the name Massilia rhizosphaerae sp. nov. is proposed, with strain NEAU-GH312T (=DSM 109722T=CCTCC AB 2019142T) as the type strain.
Funder
the national natural science foundation of china
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
17 articles.
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