Affiliation:
1. College of Life Sciences, Langfang Normal University, Langfang 065000, PR China
2. College of Biological Sciences and Biotechnology, National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing 100083, PR China
Abstract
Strain SX5T was isolated from the soil of a poultry farm in Shanxi Province, PR China. The isolate was a Gram-stain-negative, rod-shaped, non-flagellated, and yellow bacterium. Growth occurred at 20–37 °C (optimum, 28 °C), pH 6.0–10.0 (optimum, pH 8.0) and 0–1 % NaCl (optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SX5T was related to members of the genus
Luteimonas
, and close to
Luteimonas gilva
H23T (97.9 %),
Luteimonas cucumeris
Y4T (97.9 %),
Luteimonas aquatica
RIB1-20T (96.8 %),
Luteimonas notoginsengisoli
SYP-B804T (96.4 %) and
Luteimonas panaciterrae
Gsoil 068T (96.1 %). The major cellular fatty acids of strain SX5T were iso-C16 : 0, iso-C17 : 1
ω9c, iso-C15 : 0 and iso-C11 : 0 3OH. The sole isoprenoid quinone was ubiquinone Q-8, and the major polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Genome analyses revealed that strain SX5T had a genome size of 3.6 Mbp with a G+C content of 65.7 mol% and contained abundant carbohydrate-active enzyme genes and three putative distinct biosynthetic gene clusters, suggesting that it may have great potential to degrade and utilize complex biological organic matter and produce special secondary metabolites. Comparative genomic analyses clearly separated strain SX5T from the known species of the genus
Luteimonas
based on average nucleotide identity and digital DNA–DNA hybridization values below the thresholds for species delineation. Based on its phenotypic, genotypic properties and phylogenetic inference, strain SX5T represents a novel species in the genus
Luteimonas
, for which the name Luteimonas galliterrae sp. nov. is proposed. The type strain is SX5T (=GDMCC 1.2162T=KCTC 82443T=JCM 34401T).
Funder
National Natural Science Foundation of China
Fundamental Research Funds for the Central Universities at Beijing Forestry University
University–Industry Collaborative Education Program
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology