Affiliation:
1. Tianjin Agricultural University, Tianjin 300384, PR China
2. Tianjin Academy of Agricultural Sciences, Tianjin 300112, PR China
Abstract
A Gram-stain-negative, aerobic and rod-shaped bacterium, strain Y2R2T, was isolated from a saline–alkali soil sample collected from Binhai New Area, Tianjin, PR China. Growth of strain Y2R2T was observed at 10–45 °C (optimum, 30 °C), at pH 6.0–11.0 (optimum, pH 9.0) and in the presence of 0–15 % (w/v) NaCl (optimum, 9.0 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Y2R2T was affiliated with the genus
Halomonas
and showed the highest similarity to
Halomonas huangheensis
BJGMM-B45T (99.0%) and
Halomonas cupida
DSM 4740T (98.4%). The digital DNA–DNA hybridization and average nucleotide identity values of 21.0–22.8 % and 73.3–75.7 % with the closely related species
H. huangheensis
BJGMM-B45T,
H. cupida
DSM 4740T,
H. ventosae
AL12T,
H. stenophila
N12T and
H. litopenaei
SYSU ZJ2214T were lower than the threshold recommended for species discrimination.
The major respiratory quinone of strain Y2R2T was Q-9 and the major cellular fatty acids consisted of C16 : 0, C19 : 0 cyclo ω8c and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The DNA G+C content of strain Y2R2T was 57.0 mol%. On the basis of this polyphasic taxonomic study, strain Y2R2T is considered to represent a novel species of the genus
Halomonas
, for which the name Halomonas binhaiensis sp. nov. is proposed. The type strain is Y2R2T (=CGMCC 1.16974T=KCTC 72578T).
Funder
Tianjin Agricultural University Graduate Scientific Research and Innovation Project
National Natural Science Foundation of China
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
1 articles.
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