Affiliation:
1. Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, PR China
2. Frontiers Science Center for Deep Ocean Multispheres and Earth System, and College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China
3. Laboratory for Marine Ecology and Environmental Science, Qingdao Laoshan Laboratory for Marine Science and Technology, Qingdao 266071, PR China
Abstract
A Gram-stain-negative, facultative anaerobic, rod-shaped strain, named SDRW27T, was isolated from offshore seawater collected near Qingdao. Strain SDRW27T was able to grow at 16–37 °C (optimum, 28 °C), pH 6.0–9.0 (optimum, pH 6.0) and in the presence of 1–7 % (w/v) NaCl (optimum, 3 %). Phylogenetic analysis using 16S rRNA gene sequences indicated that strain SDRW27T was most closely related to
Photobacterium toruni
H01100410BT (97.89 % sequence similarity),
Photobacterium andalusiense
H01100409BT (97.89 %) and
Photobacterium leiognathi
ATCC 25521T (97.82 %). The predominant fatty acids were summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH), summed feature 8 (C18 : 1
ω7c and/or C18 : 1
ω6c) and C16 : 0. The polar lipids of strain SDRW27T comprised phosphatidylglycerol, phosphatidylinositol dimannoside, phosphatidylcholine, phosphatidylethanolamine and three unidentified lipids. The major respiratory quinone was ubiquinone-8. The G+C content was 47.71 mol%. The genome size was 5.84 Mbp, including 85 contigs with an N50 value of 223 542. The average nucleotide identity (ANI) values of SDRW27T with its three most similar strains,
P. toruni
H01100410BT,
P. andalusiense
H01100409BT and
P. leiognathi
ATCC 25521T, were 71.36, 71.58 and 72.23 %, respectively (all lower than the 95–96 % ANI threshold), and the DNA–DNA hybridization (DDH) values were 20.4, 20.8 and 20.4 % (all lower than the 70 % DDH threshold). The obtained results of polyphasic analysis demonstrate that strain SDRW27T represents a novel species, for which the name Photobacterium obscurum sp. nov. is proposed. The type strain is SDRW27T (=MCCC 1K06286T=KCTC 82892T).
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
Fundamental Research Funds for the Central Universities
Qingdao Postdoctoral Scientific Research Foundation
Postdoctoral Innovation Program of Shandong Province
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
1 articles.
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