Affiliation:
1. Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea
2. Department of Civil and Environmental Engineering, Chung-Ang University, Seoul 06974, Republic of Korea
3. Department of Animal Science and Technology, Sunchon National University, Jeonnam 57922, Republic of Korea
Abstract
A Gram-negative, moderately halophilic and facultatively aerobic bacterium, designated strain GTF13T, was isolated from a sea tidal flat. Cells were curved rods and motile by a single polar flagellum showing catalase and oxidase activities. Growth was observed at 20–37 °C, pH 5.0–8.5 and 1.0–6.0 % (w/v) NaCl. Strain GTF13T contained C16:0, summed feature 3 (comprising C16 : 1
ω6c/C16 : 1
ω7c), summed feature 8 (comprising C18 : 1
ω6c/C18 : 1
ω7c) and C12 : 0 3-OH as major fatty acids and ubiquinone-9 and ubiquinone-8 as major quinones. Phosphatidylethanolamine and two unidentified phospholipids were detected as major polar lipids. The G+C content of the genomic DNA was 59.8 mol%. Strain GTF13T was most closely related to
Simiduia agarivorans
SA1T,
Endozoicomonas montiporae
CL-33T and
Pseudomonas segetis
FR1439T, belonging to different families or orders of the class
Gammaproteobacteria
, with less than 92.0 % 16S rRNA gene sequence similarities. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain GTF13T formed a phylogenetic lineage with the family
Litoricolaceae
, but the genome-based phylogenomic tree showed that strain GTF13T formed a distinct phylogenetic lineage within the order
Oceanospirillales
. The very low 16S rRNA gene sequence similarities and distinct phylogenetic relationships, together with distinct phenotypic and chemotaxonomic properties, served to differentiate strain GTF13T from phylogenetically closely related families. Here, strain GTF13T is proposed as a novel genus and species, for which the name Aestuariirhabdus litorea gen. nov., sp. nov. is proposed, within a new family Aestuariirhabdaceae fam. nov. of the order
Oceanospirillales
. The type strain is GTF13T (=KACC 19788T=JCM 32043T).
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Reference41 articles.
1. Oceanospirillales ord. nov.
2. Oleiphilaceae fam. nov., to include Oleiphilus messinensis gen. nov., sp. nov., a novel marine bacterium that obligately utilizes hydrocarbons;Golyshin;Int J Syst Evol Microbiol,2002
3. Saccharospirillum impatiens gen. nov., sp. nov., a novel γ-Proteobacterium isolated from hypersaline Ekho Lake (East Antarctica)
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