Affiliation:
1. School of Liberal Arts and Sciences, Gateway Technical College, Kenosha, WI 53144, USA
2. Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019, USA
Abstract
A facultative anaerobic, Gram-stain-negative rod-shaped bacterium, designated RT, was isolated from the faecal material of a rabbit (Sylvilagus floridanus). The strain could not be identified using an MALDI Biotyper sirius CA System. The closest matches based on the Bruker library were members of the genera
Citrobacter
and
Pantoea
. However, the score value was in the range of no organism identification possible. Based on pairwise of 16S rRNA gene sequence analysis, the isolate was found to be a member of the family
Erwiniaceae
. The highest sequence similarities were found to the sequences of
Pantoea rodasii
LMG 26273T (98.7 %),
Leclercia adecarboxylata
NBRC 102595T (98.5 %) and
Enterobacter huaxiensis
090008T (98.4 %). Phylogenetic and whole genome analysis demonstrated that strain RT represents a novel species within the genus
Pantoea
. The predominant cellular fatty acids of strain RT were C16 : 0 and products present in summed feature 2 (C12 : 0) aldehyde, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). In silico genome analysis showed the presence of enzymes required for production of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine. The G+C content determined from the genome was 54.94 mol %. Based on biochemical, phylogenetic, genotypic and chemotaxonomic criteria, the isolate represents a novel species of the genus
Pantoea
for which the name Pantoea leporis sp. nov. is proposed. The type strain is strain RT (=CCUG 76269T=ATCC TSD-291T).
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
1 articles.
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