Streptomyces arboris sp. nov., isolated from Populus euphratica wetland soil

Abstract

A novel actinobacterial strain (TRM 68085T) was isolated from soil ofPopulus euphraticawetland. A polyphasic approach was used to study the taxonomy of TRM 68085Tand the results showed a range of phylogenetic and chemotaxonomic properties consistent with those of the genusStreptomyces. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TRM 68085Tshowed the highest similarity value toStreptomyces capitiformicae1H-SSA4T(98.6 %), and phylogenetically clustered withStreptomyces kanasensisZX01T(97.5 %) andStreptomyces ipomoeaeNBRC 13050T(97.4 %). The genomic DNA G+C content of strain TRM 68085Tbased on the genome sequence was 71.4 mol%. The levels of DNA–DNA relatedness between the genome of the isolate and its nearest phylogenetic neighbours,S. capitiformicae1H-SSA4T,S. kanasensisZX01TandS. ipomoeaeNBRC 13050T, were 19.2±0.4, 21.8±0.5 and 19.3±0.6 %, respectively. Chemotaxonomic data revealed that strain TRM 68085Tpossessed MK-9(H6) and MK-9(H8) as the predominant menaquinones.ll-Diaminopimelic acid and a small amount ofmeso-diaminopimelic acid were the diagnostic diamino acids. Ribose, xylose, glucose and galactose were the whole-cell sugars. The major cellular fatty acids were C16 : 0(25.4 %) and iso-C16 : 0(18.3 %). On the basis of these genotypic and phenotypic data, it is concluded that strain TRM 68085Trepresents a novel species of the genusStreptomyces, for which the nameStreptomyces arborissp. nov. is proposed. The type strain is TRM 68085T(=CCTCC AA2019031T=LMG 31492T).