Qingshengfaniella alkalisoli gen. nov., sp. nov., a p-hydroxybenzoate-degrading strain isolated from saline soil

Abstract

p-Hydroxybenzoate is an allelopathic compound commonly found in soil from long-term monoculture cropping systems. During the bacterial diversity analysis of saline soil, a Gram-negative, non-spore forming, non-motile, aerobic p-hydroxybenzoate-degrading bacterial strain, designated LN3S51T, was isolated from saline soil which was sampled from Tumd Right Banner, Inner Mongolia, northern China. Strain LN3S51T grew at 4–40 °C (optimum, 30 °C), pH 5.0–10.0 (optimum, pH 7.0) and 0–15 % NaCl (optimum 3.0 %). Though strain LN3S51T has the highest 16S rRNA gene similarities to Litoreibacter ponti GJSW-31T (96.0 %), the phylogenetic tree based on the 16S rRNA gene sequences showed that it clustered with Fluviibacterium aquatile SM1902T (95.8 %), Meridianimarinicoccus roseus TG-679T (93.9 %), and Phycocomes zhengii LMIT002T (93.9 %). Strain LN3S51T contained Q-10 as the major ubiquinone. Phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), diphosphatidylglycerol (DPG), an unidentified aminolipid (AL), and two unidentified lipids (L) were the major polar lipids. The major fatty acids were sum feature 8 (C18 : 1  ω7c and/or C18 : 1  ω6c), C16 : 0, C18 : 0, and C18 : 1  ω7c 11-methyl. The genome of strain LN3S51T consisted of a 2 257 066 bp chromosome and four plasmids with a 59.1 mol% of genomic DNA G+C content. The average nucleotide identity (ANI) and digital DNA–DNA hybridization score (dDDH) values of strain LN3S51T to F. aquatile SM1902T, M. roseus TG-679T, P. zhengii LMIT002T, and L. ponti GJSW-31T were 69.6, 70.9, 70.6, and 69.5 %, and 20.0, 19.5, 19.0, and 20.0 %, respectively. Based on the results of phylogenetic, chemtaxonomic and phenotypic characterization, strain LN3S51T is considered to represent a novel species in a new genus within the family Rhodobacteraceae , for which Qingshengfaniella alkalisoli gen. nov., sp. nov. is proposed. The type strain is LN3S51T (=CGMCC 1.17099T=KCTC 72457T).