Paraburkholderia flava sp. nov., isolated from cool temperate forest soil

Abstract

A Gram-stain-negative, aerobic and short rod-shaped bacterial strain, designated LD6T, was isolated from a forest soil sample in Suwon, Gyeonggi-do, Republic of Korea. Strain LD6Tgrew at 10–37 °C (optimal temperature, 28 °C), and tolerated pH 8.0 and 2 % (w/v) NaCl. Strain LD6Twas related most closely to members of the genusParaburkholderia, namelyParaburkholderia azotifigensNF2-5-3T(98.2 % 16S rRNA gene sequence similarity),P. megapolitanaA3T(97.9 %),P. ginsengiterraeDCY85T(97.9 %) andP. caribensisMWAP64T(97.7 %). The strain grew well on R2A agar, tryptone soya agar, Mueller-Hinton agar and nutrient agar. The major polar lipid profile comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, aminophospholipid and glycolipid. The major respiratory quinone was ubiquinone 8 (Q-8). The main fatty acids were C17 : 0cyclo, C16 : 0, C16 : 03-OH, C19 : 0cyclo ω8cand C12 : 0. The DNA G+C content of the isolated strain based on the whole genome sequence was 63.4 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain LD6Tand its reference type strains ranged from 80.3 to 82.4%, and from 23.7 to 33.7%, respectively. Based on phenotypic, chemotypic and genotypic evidence, strain LD6Tcould be differentiated phylogenetically and phenotypically from the recognized species of the genusParaburkholderia. Therefore, strain LD6Tis considered to represent a novel species, for which the nameParaburkholderia flavasp. nov. is proposed. The type strain is LD6T(=KACC 21387T=JCM 33640T).