Affiliation:
1. University of Science and Technology (UST), 217 Gajeong-ro, Yuseong, Daejeon 34113, Republic of Korea
2. Biological Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, 181 Ipsingil, Jeongeup, Jeonbuk 56212, Republic of Korea
Abstract
A Gram-stain-positive, non-spore-forming, yellow-pigmented, non-motile, non-flagellated, facultative anaerobic and rod-shaped bacterial strain, designated BI34T, was isolated from the gut of the larva of Protaetia brevitarsis seulensis. Strain BI34T grew at 15–40 °C (optimum, 37 °C), at pH 6.5–9.0 (optimum, pH 7.5) and in the presence of 0–7 % (w/v) NaCl (optimum, 2 %). Based on the results of 16S rRNA gene sequence analysis, strain BI34T belonged to the phylum
Actinobacteria
and was closely related to
Cellulosimicrobium funkei
NBRC 104118T (99.3 %),
Cellulosimicrobium cellulans
NBRC 15516T (99.1 %),
Cellulosimicrobium composti
BIT-GX5T (99.0 %),
Cellulosimicrobium fucosivorans
SE3T (99.0 %),
Cellulosimicrobium marinum
NBRC 110994T (98.4 %) and
Cellulosimicrobium terreum
DS-61T (97.0 %). The genome to genome relatedness of the average nucleotide identity (ANI) and the digital DNA–DNA hybridization (dDDH) values calculated by the Genome-to-Genome Distance Calculator between strain BI34T and its related species mentioned above were lower than the threshold of 95 and 70 % for speciation, respectively. The predominant menaquinone of strain BI34T contained MK-9(H4), and the major fatty acids were anteiso-C15 : 0, C16 : 0 and anteiso-C17 : 0. Strain BI34T had diphosphatidylglycerol and phosphatidylglycerol as major polar lipids. The whole-cell sugars were galactose, glucose and ribose, and the cell-wall peptidoglycan contained lysine, alanine, aspartic acid and glutamic acid. The DNA G+C content of strain BI34T was 73.8 mol%. The difference in physiological and biochemical characteristics and the below-threshold values of genome-to-genome relatedness indicate that strain BI34T represents a novel species in the genus
Cellulosimicrobium
, for which the name Cellulosimicrobium protaetiae sp. nov. is proposed. The type strain is BI34T (=KCTC 49302T=NBRC 114073T).
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
7 articles.
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