Adhaeribacter arboris sp. nov., and Adhaeribacter pallidiroseus sp. nov., novel bacteria isolated from birch tree (Betula platyphylla)

Abstract

Two Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterial strains, designated as HMF7605T and HMF7616T, were isolated from birch tree, in Yong-in, Republic of Korea. Strains HMF7605T and HMF7616T exhibited the highest 16S rRNA gene sequence similarities of 95.9 and 97.5 % to Adhaeribacter swui 17mud1-7T, 97.9 % between themselves. The values of average nucleotide identity and in silico DNA–DNA hybridization between strains HMF7605T and HMF7616T were 77.6 and 22.0 %, respectively. Phylogenetic analysis of the 16S rRNA gene sequences of the two strains revealed that they belonged to the genus Adhaeribacter within the family Hymenobacteraceae . The predominant fatty acids of both strains were iso-C15 : 0, summed feature 3 (comprising C16 : 1  ω7c and/or C16 : 1  ω6c), C16 : 1  ω5c and summed feature 4 (comprising iso-C17 : 1 I and/or anteiso-C17 : 1 B). The both strains contained menaquinone-7 as the only isoprenoid quinone. The major polar lipid profiles of the two strains were similar with phosphatidylethanolamine, one unidentified aminophosphoglycolipid, one unidentified glycolipid and three unidentified polar lipids. The DNA G+C contents of strains HMF7605T and HMF7616T were 42.0 and 42.8 mol%, respectively. Based on the results of the phenotypic, genotypic, chemotaxonomic and phylogenetic investigation, two novel species, Adhaeribacter arboris sp. nov. and Adhaeribacter pallidiroseus sp. nov. are proposed. The type strains are HMF7605T (=KCTC 62465T=NBRC 113228T) and HMF7616T (=KCTC 62466T=NBRC 113229T), respectively.