Polymorphobacter arshaanensis sp. nov., containing the photosynthetic gene pufML, isolated from a volcanic lake

Abstract

An aerobic, Gram-stain-negative, yellowish, rod-shaped bacterium, designated DJ1R-1T, was isolated from water sample from a volcanic lake, located on Da Hinggan Ling Mountain, PR China. Growth of DJ1R-1T optimally occurred at pH 7.0, at 22–25 °C and with 0–0.5 % (w/v) NaCl concentration. Phylogenetic analysis of 16S rRNA gene sequences indicated that DJ1R-1T was clustered into the genus Polymorphobacter, and showed 96.5 %, 95.9 % and 95.6 % similarities to Polymorphobacter fuscus D40PT, Polymorphobacter multimanifer 262-7T and Polymorphobacter glacialis B555-2T, respectively. The predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid, three unidentified aminolipids and one unidentified phospholipid. The major fatty acids were summed feature 8 (C18 : 1ω7c / C18 : 1ω6c, 40.0 %), summed feature 3 (C16 : 1ω7c / C16 : 1ω6c, 25.6 %) and C16 : 0 (13.7 %). The respiratory quinone was ubiquinone-10. The DNA G+C content was 65.0 % according to the genomic sequencing results. On the basis of the results of the phylogenetic analysis, physiological and biochemical properties comparisons, DJ1R-1T was proposed to represent a novel species of the genus Polymorphobacter , with the name Polymorphobacter arshaanensis. The type strain is DJ1R-1T (=CGMCC 1.13788T=KCTC 72014T).