Haemophilus seminalis sp. nov., isolated from human semen

Author:

Zheng Min-ling1,Li Liang-hui1,Liu Bin2,Lin Yu-bo3ORCID,Zhang Xiao-tuan2,Chen Cha41,Qu Ping-Hua14,Zeng Jian2

Affiliation:

1. The Second Clinic Medical College, Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China

2. The Second Hospital, University of South China, Hengyang 421001, PR China

3. Department of Clinical Laboratory, Wuchuan Maternity and Child Health Care Hospital, Wuchuan 524500, PR China

4. Department of Clinical Laboratory, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou 510006, PR China

Abstract

Two Haemophilus -like isolates with similar biochemical characteristics, designated strains SZY H1T and SZY H2, were isolated from human semen specimens. Cells were Gram-negative, non-motile, non-acid-fast, pleomorphic rods or coccobacilli. The major fatty acids (>10 %) were C16 : 0, C14 : 0, iso-C16 : 0 and/or C14 : 0 3-OH and C16 : 1 ω6c and/or C16 : 1 ω7c. The polar lipids were determined to be phosphatidylethanolamine, phosphatidylglycerol, an unidentified phospholipid, an unidentified aminophospholipid, two unidentified polar lipids and four unidentified aminolipids. The major polyamine was found to be cadaverine. The near-full-length (1462 nt) 16S rRNA gene sequences analysis showed the two isolates were nearly identical (>99.8 %), and closely matched Haemophilus haemolyticus ATCC 33390T with 98.9–99.1 % sequence similarities. Phylogenetic analysis based on 16S rRNA gene sequences and concatenation of 30 protein markers also revealed that the isolates clustered together with H. haemolyticus ATCC 33390T, and formed a distinct lineage well separated from the other members of the genus Haemophilus . Further, the average nucleotide identity values between the two isolates and their related species were below the established cut-off values for species delineation (95 %). Based on these findings, the two isolates are considered to represent a new species of the genus Haemophilus , for which name Haemophilus seminalis sp. nov. is proposed. The type strain is SZY H1T (=NBRC 113782T=CGMCC 1.17137T).

Funder

Research Fund for Science and Technology Planning Project of Guangdong Province, China

Publisher

Microbiology Society

Subject

General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology

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