Affiliation:
1. School of Marine Sciences, China University of Geosciences, Beijing 100083, PR China
2. Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, PR China
3. School of Marine Biology, Xiamen Ocean Vocational College, Xiamen, PR China
Abstract
A novel mesophilic, chemolithoautotrophic, hydrogen-oxidizing bacterium, designated strain ST1-3T, was isolated from mud sediment samples collected from mangroves in Jiulong River estuary. The cells were Gram-stain-negative, non-motile and rod-shaped. The temperature, pH and salinity ranges for growth of strain ST1-3T were 4–45 °C (optimum, 35 °C), pH 5.0–8.5 (optimum, pH 7.0) and 0–8.0 % (w/v) NaCl (optimum, 4.0 %). The isolate was an obligate chemolithoautotroph capable of growth using hydrogen as the only energy source, and molecular oxygen, thiosulphate and elemental sulphur as electron acceptors. The major cellular fatty acids of strain ST1-3T were summed feature 3 (C16 : 1
ω7c and/or C16 : 1
ω6c), C16 : 0 and summed feature 8 (C18 : 1
ω7c). The major polar lipids were phosphatidylethanolamine, phosphatidyldimethyl ethanolamine and phosphatidylglycerol. The respiratory quinone was menaquinone-6. The genomic DNA G+C content was 43.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences and core genes showed that the novel isolate belonged to the genus
Sulfurovum
and was most closely related to
Sulfurovum lithotrophicum
42BKTT (94.7 % sequence identity). The average nucleotide identity and digital DNA–DNA hybridization values between ST1-3T and
S. lithotrophicum
42BKTT were 74.6 and 16.3 %, respectively. On the basis of the phenotypic, phylogenetic and genomic data presented here, strain ST1-3T represents a novel species of the genus
Sulfurovum
, for which the name Sulfurovum mangrovi sp. nov. is proposed, with the type strain ST1-3T (=MCCC M25234T=KCTC 25639T).
Funder
National Natural Science Foundation of China
China Ocean Mineral Resources Research and Development Association
China Postdoctoral Science Foundation
Scientific Research Foundation of Third Institute of Oceanography
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology