Affiliation:
1. College of Life Science, University of Chinese Academy of Sciences, Beijing, PR China
2. State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, PR China
Abstract
A novel Gram-stain-negative, short-rod-shaped, orange-pigmented bacterial strain, designated L-1–4 w-11T, was isolated from Baiyang Lake in China. The strain grew at 15–35 °C (optimum 30 °C) and pH 7–8 (optimum pH 7) in TSA medium. The predominant polar lipids of strain L-1–4 w-11T were sphingoglycolipid, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and three unidentified lipids; the major cellular fatty acids were C17 : 1ω6c and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c); and the major respiratory quinone was ubiquinone 10 (Q-10). Strain L-1–4 w-11T showed the highest 16S rRNA gene sequence similarity to
Sphingomonas japonica
JCM 15438T (98.3 %) and
S. spermidinifaciens
GDMCC 1.657T (98.0 %). The draft genome size of strain L-1–4 w-11T was 3.3 Mbp, and the G+C content was 67.8 mol%. Digital DNA–DNA hydridization and average nucleotide identity values between the genome sequences of strain L-1–4 w-11T and
S. spermidinifaciens
GDMCC 1.657T (76.9 and 21.0 %),
S. japonica
JCM 15438T (76.0 and 19.9 %) and
S. paucimobilis
CGMCC 1.12825T (72.8 and 19.6 %) were far below the thresholds for prokaryotic conspecific assignment. With the evidence from the phylogenetic, chemotaxonomic and genotypic analyses, we propose that strain L-1–4 w-11T represents a novel
Sphingomonas
species with the name S. baiyangensis sp. nov. The type strain is L-1–4 w-11T (=CGMCC 1.13572T=JCM 33962T).
Funder
Science and Technology Basic Resources Investigation Project
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology