Affiliation:
1. State Key Laboratory of Microbial Technology, Institute of Microbiology Technology, Shandong University, Qingdao 266237, PR China
Abstract
A Gram-stain-negative, strictly aerobic, non-motile, orange-coloured bacterium, designated YR1-1T, was isolated from a soil sample collected from the Yellow River Delta wetlands (PR China). Growth was observed at a salinity of 1.0–15.0 % NaCl, 4–45 °C and pH 6.0–9.0. The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that YR1-1T represented a member of the genus
Psychroflexus
, with the highest sequence similarity to
Psychroflexus sediminis
YIM-C238T (97.9 %), followed by
Psychroflexus aestuariivivens
(97.1 %) and
Psychroflexus torquis
(96.4 %). The average nucleotide identity and digital DNA–DNA hybridization values between YR1-1T and other closely related type strains of species of the genus
Psychroflexus
were 68.7–86.3% and 17.8–30.9 %. The genome of the strain was 2 899 374 bp in length with 39.8 % DNA G+C content. The predominant fatty acids (>10 %) were iso-C15 : 0 and anteiso-C15 : 0. The major respiratory quinone was menaquinone-6 (MK-6) and the major polar lipids were phosphatidylethanolamine, phospholipid, diphosphatidylglycerol, two unidentified aminolipids and four unidentified lipids. The combined genotypic and phenotypic data indicate that YR1-1T represents a novel species within the genus
Psychroflexus
, for which the name Psychroflexus aurantiacus sp. nov., is proposed. The type strain is YR1-1T (=KCTC 72794T=CGMCC 1.17458T).
Funder
Special investigation on scientific and technological basic resources
the special national project on investigation of basic resources of China
the National Natural Science Foundation of China
the Key Program of Shandong Natural Science Foundation
the National Key Research and Development Programs of China
National Natural Science Foundation of China
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
3 articles.
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