Affiliation:
1. College of Marine Life Sciences, and Institute of Evolution & Marine Biodiversity, Ocean University of China, Qingdao 266003, PR China
2. Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, PR China
3. Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao 266100, PR China
Abstract
A Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacterium, designated ZYF650T, was isolated from the hadal seawater (9600 m) of the Mariana Trench. Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that ZYF650T formed a lineage within the family
Alteromonadaceae
that was distinct from the most closely related species
Marinobacter mobilis
and
Marinobacter nitratireducens
with 16S rRNA gene sequences similarities of 98.0 and 97.7 %, respectively. Strain ZYF650T showed average nucleotide identity values of 75.7 % with
Marinobacter hydrocarbonoclasticus
, 73.3 % with
Marinobacter mobilis
and 79.3 % with
Marinobacter nitratireducens
, and DNA–DNAhybridization values of 21.5, 21.3 and 22.0 % with
M. hydrocarbonoclasticus
,
M. mobilis
and
M. nitratireducens
, respectively, which were lower than the threshold for species delineation. Strain ZYF650T grew with 0–14 % (w/v) NaCl (optimum, 7–8 %) at a temperature range of 10–45 °C (optimum, 28 °C) and pH 6.0–9.5 (optimum, pH 7.0–8.0). The sole respiratory quinone was ubiquinone-9 (Q-9). The polar lipids in ZYF650T comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, three unidentified polar lipids, two unidentified aminolipids and two phospholipids. The predominant fatty acids (more than 10 % of total fatty acids) were C18 : 1 ω9c (21.9 %), C16 : 0 (21.7 %), C12 : 0 3-OH (14.0 %), C16 : 1 ω9c (13.2 %) and C12 : 0 (12.2 %). The DNA G+C content of strain ZYF650T was 55.6 %. On the basis of polyphasic taxonomic analysis, strain ZY650T is considered to represent a novel specie of the genus
Marinobacter
in the family
Alteromonadaceae
, for which the name Marinobacter salinexigens sp. nov. is proposed. The type strain is ZYF650T (=JCM 33013T=MCCC 1K03552T).
Funder
National Natural Science Foundation of China
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
7 articles.
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