Affiliation:
1. Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, 99, Daehak-ro, Yuseong, Daejeon 34134, Republic of Korea
Abstract
A mycolic acid-containing actinobacterium designated strain MMS17-SY073T was isolated from island soil. The isolate showed best growth at 25 °C, pH 6, and 0 % (w/v) NaCl. The phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MMS17-SY073T belongs to the genus
Gordonia
, and is mostly related to the type strains of
Gordonia soli
(98.5 % sequence similarity),
Gordonia polyisoprenivorans
(98.1%), and
Gordonia hankookensis
(97.8%). The genome-based comparisons showed a clear distinction between the strain and the two neighbouring species,
G. soli
and
G. polyisoprenivorans
, with the average nucleotide identities (ANI) of 75.8 and 76.3 %, respectively. Notably, the genome of strain MMS17-SY073T was the largest in total stretch and gene counts among the complete genomes of
Gordonia
, and contained a number of biosynthetic gene clusters for secondary metabolites, in particular those for non-ribosomal peptide synthetases. The major polar lipids were diphosphatidyl glycerol (DPG), phosphatidyl glycerol (PG), phosphatidyl ethanolamine (PE), phosphatidyl inositol (PI) and phosphatidyl inositol mannoside (PIM). The isoprenoid quinone was MK-9(H2), and the main fatty acids were C16 : 0 (30.2%) and 10-methyl-C18 : 0 (33.7%). The whole cell hydrolysates contained galactose, arabinose, and meso-diaminopimelic acid. The DNA G+C content was 67.4 mol%. Based on phenotypic, chemotaxonomic and genetic analysis, strain MMS17-SY073T should be classified as a new species of the genus
Gordonia
, for which the name Gordonia insulae sp. nov. is proposed (type strain=MMS17-SY073T=KCTC 49257T=JCM 33277T).
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
10 articles.
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