Affiliation:
1. College of Biological Engineering, Henan University of Technology, Zhengzhou 450001, Henan Province, PR China
2. State Key Laboratory of Motor Vehicle Biofuel Technology, Nanyang 473000, Henan Province, PR China
Abstract
A novel bacterial strain, designated NS28T, was isolated from interfacial sediment sampled at Taihu Lake, PR China. Cells were rod-shaped, Gram-negative, aerobic and non-motile on Reasoner's 2A medium. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NS28T was most closely related to species from the genus
Dyadobacter
, with 98.4 and 96.0 % 16S rRNA gene sequence similarity to its closest phylogenetic neighbours
Dyadobacter sediminis
CGMCC 1.12895T and
Dyadobacter luticola
CCTCC AB 2017091T, respectively. MK-7 was the only cellular menaquinone. The major fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0 and C16 : 1ω5c. The major polar lipids were phosphatidylethanolamine, one phospholipid, one aminolipid, one lipid and two unidentified lipids. Genomic analysis of strain NS28T indicated that the total genome size was 6 477 094 bp with a G+C content of 44.8 mol%, 5380 protein-coding genes, 79 contigs and an N50 length of 299584 bp. On the basis of the genomic DNA sequence, the average nucleotide identity values were 90.5 and 74.1 % with
D. sediminis
CGMCC 1.12895T and
D. luticola
CCTCC AB 2017091T, respectively. Digital DNA-DNA hybridization results of strain NS28T with
D. sediminis
CGMCC 1.12895T and
D. luticola
CCTCC AB 2017091T were 40.9 and 18.6 %, respectively. Based on the phenotypic, chemotaxonomic, phylogenetic and genome sequence data presented here, it is proposed that strain NS28T represents a novel species of the genus
Dyadobacter
for which the name Dyadobacter flavalbus is proposed . The type strain is NS28T (=NBRC 113854T=MCCC 1K03764T).
Funder
Key Scientific Research Project of Colleges and Universities in Henan Province
National Natural Science Foundation of China
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献