Affiliation:
1. The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, PR China
2. The Key Laboratory of Microbial Resources of Xuzhou City, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, PR China
Abstract
A Gram-positive, aerobic actinomycete, designated strain KLBMP 9356T, was isolated from weathered potash tailings soil sampled in Xuzhou, Jiangsu Province, PR China. The colonies were cream-coloured, convex and rounded. The optimal growth conditions of strain KLBMP 9356T were 1 % (w/v) NaCl, 28 °C and pH 7. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KLBMP 9356T showed the highest similarity to
Nocardioides zhouii
CGMCC 1.11084T (98.9 %) and
Nocardioides glacieisoli
CGMCC 1.11097T (98.7 %). Results from two tree-making algorithms supported the position that strain KLBMP 9356T forms a stable clade with
N. zhouii
CGMCC 1.11084T and
N. glacieisoli
CGMCC 1.11097T. Strain KLBMP 9356T exhibited low digital DNA-DNA hybridization values with
N. zhouii
CGMCC 1.11084T (27.6 %) and
N. glacieisoli
CGMCC 1.11097T (31.4 %). The average nucleotide identity values between strain KLBMP 9356T and
N. zhouii
CGMCC 1.11084T and
N. glacieisoli
CGMCC 1.11097T were 83.8% and 85.9%, respectively. The peptidoglycan in the cell wall of the novel strain was ll-2,6-diaminopimelic acid and the predominant menaquinone was MK-8(H4). The major fatty acids (>10 %) were C17:1ω8c and C18:1ω9c. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, lyso-phospatidylglycerol and phosphatidylinositol. The genomic DNA G+C content was 71.6 mol%. Based on its morphological, chemotaxonomic and phylogenetic characteristics, strain KLBMP 9356T represents a novel species of the genus
Nocardioides
, for which the name Nocardioides potassii sp. nov. is proposed. The type strain is KLBMP 9356T (=CGMCC 4.7738T=NBRC 115493T).
Funder
Graduate Research and Innovation Projects of Jiangsu Province
Key Research Plan of Modern Agriculture of Xuzhou City
Young Scientists Fund
the State Key Laboratory of Environmental Geochemistry
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology