Pseudokineococcus galaxeicola sp. nov., isolated from mucus of a stony coral

Abstract

A Gram-stain-positive, aerobic, motile, coccus-shaped bacterium, designated strain SCSIO 13315T, was isolated from mucus of the coral Galaxea sp. collected from Luhuitou fringing reef (Sanya, Hainan, PR China). Analysis of the 16S rRNA gene sequence showed that strain SCSIO 13315T exhibits 95.5 % 16S rRNA gene sequence similarity to Pseudokineococcus basanitobsidens SKC1-2T, 95.8 % to Pseudokineococcus lusitanus T2A-S27T and 96.4 % to Pseudokineococcus marinus KST3-3T. Results of phylogenetic analysis suggested that strain SCSIO 13315T was a member of the genus Pseudokineococcus . The DNA G+C content of strain SCSIO 13315T was 73.5 %. Chemotaxonomic assessment of strain SCSIO 13315T showed that the menaquinones were MK-8(H4) and MK-9(H2). The main cellular fatty acids were anteiso-C15 : 0, C16 : 0 and summed feature 3 (C16 : 1  ω7c/C16 : 1  ω6c). The polar lipids present were diphosphatidylglycerol, phosphatidylglycerol, two unknown phospholipids, four unidentified aminolipids and one unidentified lipid. Based on the phylogenetic and phenotypic analysis, it was evident that strain SCSIO 13315T represents a novel species of the genus Pseudokineococcus , for which the name Pseudokineococcus galaxeicola sp. nov. is proposed. The type strain is SCSIO 13315T (=NBRC 109944T=DSM 27812T).