Affiliation:
1. College of Fisheries, Henan Normal University, Xinxiang 453007, PR China
2. Synthetic Biology Engineering Lab of Henan Province, College of Life Sciences and Technology, Xinxiang Medical University, Xinxiang 453003, PR China
Abstract
A novel actinobacterium, designated strain CFH S0261T, was isolated from a sediment sample of the Yellow River. The taxonomic position of the strain was investigated by using a polyphasic approach. Cells of strain CFH S0261T were Gram-reaction-positive, aerobic, non-motile. Growth occurs at 15–37 °C, pH 6.0–8.0 and with 0–9.0 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CFH S0261T was a member of the genus
Amycolatopsis
. The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of
Amycolatopsis niigatensis
LC11T (98.95 %),
Amycolatopsis echigonensis
LC2T (98.81 %) and
Amycolatopsis albidoflavus
IMSNU 22139T (98.73 %). The whole-genome of CFH S0261T showed a G+C content of 69.5 mol%. The ANI values and in silico DDH values between CFH S0261T and the other species of the genus
Amycolatopsis
were found to be low (ANIb <90.61 % and DDH <53.40 %). The cell wall diamino acid in the peptidoglycan of strain CFH S0261T was meso-diaminopimelic acid and the whole-cell hydrolysate comprised arabinose, galactose, glucose, rhamnose and ribose. The predominant menaquinone was MK-9(H4). The major cellular fatty acids were C16 : 0, iso-C15 : 0 and iso-C16 : 0. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and four unidentified glycolipids. On the basis of phenotypic, genotypic and phylogenetic data, strain CFH S0261T represents a novel species of the genus
Amycolatopsis
, for which the name Amycolatopsis nivea sp. nov. is proposed. The type strain is CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T).
Funder
Natural Science Foundation of China
Innovation Scientists and Technicians Troop Construction Projects of Henan Province
Henan Province University youth researcher support project
Doctor Scientific Research Fund of Xinxiang Medical University
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
3 articles.
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