Affiliation:
1. Laboratory of Molecular Environmental Microbiology, Department of Environmental Science and Ecological Engineering, Korea University, Seoul 02841, Republic of Korea
2. National Institute of Biological Resources, Incheon, 22689, Republic of Korea
Abstract
A novel cold-tolerant bacterium, designated strain YJ56T, was isolated from Antarctic soil collected from the Cape Burk area. Phylogenetic analysis through 16S rRNA gene sequence similarity revealed that strain YJ56T was most closely related to the genus
Pseudarthrobacter
, including
Pseudarthrobacter oxydans
DSM 20119T (99.06 % similarity),
Pseudarthrobacter polychromogenes
DSM 20136T (98.98 %) and
Pseudarthrobacter sulfonivorans
ALLT (98.76 %). The genome size (5.2 Mbp) of strain YJ56T was the largest among all the published genomes of
Pseudarthrobacter
type strains (4.2–5.0 Mbp). The genomic G+C content of strain YJ56T (64.7 mol%) was found to be consistent with those of other
Pseudarthrobacter
strains (62.0–71.0 mol%). The average nucleotide identity and average amino acid identity values between strain YJ56T and
P. sulfonivorans
ALLT were estimated at 84.1 and 84.2 %, respectively. The digital DNA–DNA hybridization value between the two strains was calculated to be 28.0 %. This rod-shaped and obligate aerobic strain exhibited no swimming or swarming motility. It had catalase activity but no oxidase activity. Cells grew at 4–28 °C (optimum, 13 °C) and pH 5.0–11.0 (optimum, pH 7.0) and with 0–6.0 % (w/v) NaCl (optimum, 0%) in Reasoner's 2A medium. MK-9 (H2) was the sole menaquinone. Two-dimensional TLC results revealed that the primary polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two glycolipids and phosphatidylinositol. Fatty acid methyl ester analysis showed that anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0, C16 : 0 and iso-C16 : 0 were the major cellular fatty acids in strain YJ56T. Based on phenotypic and genotypic characteristics, strain YJ56T represents a novel species of the genus
Pseudarthrobacter
, and thus the name Pseudarthrobacter psychrotolerans sp. nov is proposed. The type strain is YJ56T (=JCM 33881T=KACC 21510T).
Funder
National Institute of Biological Resources
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
18 articles.
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