Affiliation:
1. Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518057, PR China
2. Archaeal Biology Center, Institute for Advanced Study, Shenzhen University, Shenzhen 518057, PR China
3. Shenzhen Xbiome Biotech Co. Ltd., Shenzhen, Guangdong 518058, PR China
Abstract
A Gram-stain-negative, strictly aerobic, motile, rod-shaped bacterium, FT117T, was isolated from mangrove sediment collected in Shenzhen, Guangdong, PR China. Growth occurred at 20–45 °C (optimum, 40 °C), pH 6–10 (optimum, 8) and in the presence of 0–5 % NaCl (optimum, 0 %). The results of 16S rRNA gene sequence analysis indicated that the identity between FT117T and
Zeimonas arvi
CC-CFT501T was the highest (98.7 %), followed by
Quisquiliibacterium transsilvanicum
JCM 31785T (93.6 %),
Lautropia mirabilis
CCUG 34794T (93.6 %) and
Pandoraea eparura
LMG 31012T (93.5 %). The main fatty acids (>10 %) were C16 : 0 (35.8 %), cyclo-C17 : 0 (18.5 %) and summed feature 3 (18.1 %). The polar lipids contained phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids, two unidentified aminophospholipids and one unidentified lipid. The sole respiratory quinone was ubiquinone-8 (Q-8). Its DNA G+C content was 70.6 % (data from the genome sequence) and the estimated genome size was 3.860 Mb. The average nucleotide identity values between the FT117T genome and the genomes of
Z. arvi
CC-CFT501T,
Q. transsilvanicum
JCM 31785T,
L. mirabilis
CCUG 34794T and
Paraburkholderia strydomiana
LMG 31012T were 85.4 %, 76.4 %, 73.0 % and 71.3 %, and the digital DNA–DNA hybridization values were 29.1 %, 21.0 %, 20.3 % and 19.1 %, respectively. The phylogenetic, phenotypic and chemotaxonomic differences between FT117T and its phylogenetic relatives indicate that FT117T should be regarded as representing a novel species within the genus
Zeimonas
, for which the name Zeimonas sediminis sp. nov. is proposed. The type strain is FT117T (=KCTC 92314T = MCCC 1K07396T).
Funder
National Science and Technology Fundamental Resources Investigation Program of China
Innovation Team Project of Universities in Guangdong Province
Shenzhen Science and Technology Program
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology