Roseomonas harenae sp. nov., from desert gravel soil

Abstract

A Gram-stain-negative, aerobic, non-motile, pink-pigmented, coccus bacterium, designated CPCC 101081T, was isolated from a gravel soil sample collected from Badain Jara desert, PR China. Growth of the isolate occurred at 10–37 °C and pH 5.0–8.0, with optimal growth at 28–32 °C and pH 7.0, respectively. The major cellular fatty acids were summed feature 8 (C18:1ω7c/C 18:1ω6c), summed feature 3 (C 16:1ω6c/C16:1ω7c) and C18:12-OH. Q-10 was detected as the main respiratory quinone. Diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unidentified phospholipid, an amino-containing lipid and an unidentified glycophospholipid were examined in the polar lipids extraction. The 16S rRNA gene sequence comparison of strain CPCC 101081T with the available sequences in the GenBank database showed that the isolate was closely related to members of the genus Rosenomonas, with the highest similarity to Roseomonas rosea DSM 14916T (97.4 %). In the phylogenetic trees based on 16S rRNA gene sequences and the core genomes, strain CPCC 101081T was included within the clade of the genus Roseomonas , representing a species level, with the closest neighbor of R. rosea DSM 14916T . The genomic DNA G+C content was 68.7 mol%. The average nucleotide identity and the digital DNA–DNA hybridization values between strain CPCC 101081T and the related type strains of the genus Roseomonas were all far lower than the cut-off values for definition species. On the basis of above phenotypic and genotypic characteristics, strain CPCC 101081T is proposed to represent a novel species of the genus Roseomonas with the name Roseomonas harenae sp. nov. strain CPCC 101081T (=KCTC 62852T=NBRC 113512T) is the type strain of the species.