Limosilactobacillus balticus sp. nov., Limosilactobacillus agrestis sp. nov., Limosilactobacillus albertensis sp. nov., Limosilactobacillus rudii sp. nov. and Limosilactobacillus fastidiosus sp. nov., five novel Limosilactobacillus species isolated from the vertebrate gastrointestinal tract, and proposal of six subspecies of Limosilactobacillus reuteri adapted to the gastrointestinal tract of specific vertebrate hosts-Reference-Cited by-同舟云学术

Limosilactobacillus balticus sp. nov., Limosilactobacillus agrestis sp. nov., Limosilactobacillus albertensis sp. nov., Limosilactobacillus rudii sp. nov. and Limosilactobacillus fastidiosus sp. nov., five novel Limosilactobacillus species isolated from the vertebrate gastrointestinal tract, and proposal of six subspecies of Limosilactobacillus reuteri adapted to the gastrointestinal tract of specific vertebrate hosts

Published:2021-02-01 Issue:2 Volume:71 Page:
ISSN:1466-5026
Container-title:International Journal of Systematic and Evolutionary Microbiology
language:en
Short-container-title:

Author:

Li Fuyong¹^ORCID,Cheng Christopher C.²^ORCID,Zheng Jinshui³,Liu Junhong¹^ORCID,Quevedo Rodrigo Margain¹^ORCID,Li Junjie¹,Roos Stefan⁴^ORCID,Gänzle Michael G.¹^ORCID,Walter Jens²⁵¹^ORCID

Affiliation:

1. Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, T6G 2E1, Canada

2. Department of Biological Sciences, University of Alberta, Edmonton, Alberta, T6G 2E1, Canada

3. Huazhong Agricultural University, State Key Laboratory of Agricultural Microbiology, Hubei Key Laboratory of Agricultural Bioinformatics, Wuhan, Hubei, 430070, PR China

4. Department of Molecular Sciences, Uppsala BioCenter, Swedish University of Agricultural Sciences, Uppsala, 750 07, Sweden

5. APC Microbiome Ireland, School of Microbiology, and Department of Medicine, University College Cork, Cork, T12 YT20, Ireland

Abstract

Ten strains, BG-AF3-AT, pH52_RY, WF-MT5-AT, BG-MG3-A, Lr3000T, RRLNB_1_1, STM3_1T, STM2_1, WF-MO7-1T and WF-MA3-C, were isolated from intestinal or faecal samples of rodents, pheasant and primate. 16S rRNA gene analysis identified them as

Limosilactobacillus reuteri

. However, average nucleotide identity and digital DNA–DNA hybridization values based on whole genomes were below 95 and 70 %, respectively, and thus below the threshold levels for bacterial species delineation. Based on genomic, chemotaxonomic and morphological analyses, we propose five novel species with the names Limosilactobacillus balticus sp. nov. (type strain BG-AF3-AT=DSM 110574T=LMG 31633T), Limosilactobacillus agrestis sp. nov. (type strain WF-MT5-AT=DSM 110569T=LMG 31629T), Limosilactobacillus albertensis sp. nov. (type strain Lr3000T=DSM 110573T=LMG 31632T), Limosilactobacillus rudii sp. nov. (type strain STM3_1T=DSM 110572T=LMG 31631T) and Limosilactobacillus fastidiosus sp. nov. (type strain WF-MO7-1T=DSM 110576T=LMG 31630T). Core genome phylogeny and experimental evidence of host adaptation of strains of

L. reuteri

further provide a strong rationale to consider a number of distinct lineages within this species as subspecies. Here we propose six subspecies of

L. reuteri

subsp. kinnaridis subsp. nov. (type strain AP3T=DSM 110703T=LMG 31724T),

L. reuteri

subsp. porcinus subsp. nov. (type strain 3c6T=DSM 110571T=LMG 31635T),

L. reuteri

subsp. murium subsp. nov. (type strain lpuph1T=DSM 110570T=LMG 31634T),

L. reuteri

subsp.

reuteri

subsp. nov. (type strain F 275T=DSM 20016T=ATCC 23272T),