Affiliation:
1. Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China
2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China
Abstract
A novel actinomycete, designated strain NEAU-Ht49T, was isolated from sea sand sampled in Sanya and characterized by using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-Ht49T was most closely related to
Actinomadura rhizosphaerae
SDA37T (98.8 %),
Actinomadura logoneensis
NEAU-G17T (98.6 %),
Actinomadura oligospora
ATCC 43269T (98.6 %) and
Actinomadura gamaensis
NEAU-Gz5T (98.6 %). The results of phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain NEAU-Ht49T formed a cluster with
A. rhizosphaerae
SDA37T,
A. logoneensis
NEAU-G17T,
A. oligospora
ATCC 43269T,
A. gamaensis
NEAU-Gz5T and
Actinomadura rupiterrae
CS5-AC15T (96.4 %). Meso-diaminopimelic acid was detected in its cell walls and glucose, madurose, mannose and ribose were detected in whole-cell hydrolysate. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositolmannoside and two unidentified lipids. The majoy menaquinone was MK-10(H6) and the minor menaquinones were MK-9(H4) and MK-9(H8). The major fatty acids were C16 : 0, C18 : 1ω9c, 10-methyl C18 : 0 and iso-C16 : 0. Moreover, morphological and chemotaxonomic characteristics of properties of strain NEAU-Ht49T also confirmed the affiliation of the isolate to the genus
Actinomadura
. However, DNA–DNA relatedness, physiological and biochemical data showed that strain NEAU-Ht49T could be distinguished from its closest relatives. Therefore, strain NEAU-Ht49T represents a novel species of the genus
Actinomadura
, for which the name Actinomadura harenae sp. nov. is proposed, with strain NEAU-Ht49T (=CGMCC 4.7499T=JCM 32659T) as the type strain.
Funder
the National Natural Science Foundation of China
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
2 articles.
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