Affiliation:
1. Department of Microbiology, Chung-Ang University College of Medicine, Seoul 06974, Republic of Korea
2. Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, Republic of Korea
3. Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, Republic of Korea
4. Department of Microbiology, Faculty of Science, Prince of Songkla University, Songkhla 90112, Thailand
Abstract
A Gram-stain-negative, non-pigmented, non-spore-forming, motile, strictly aerobic bacterial strain, designated CAU 1492T, was isolated from a coastal sand dune and its taxonomic position was examined using a polyphasic approach. Cells of strain CAU 1492T grew optimally at 30 °C, pH 7.0 and in 3 % (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence of CAU 1492T showed that it formed a distinct lineage within the family
Rhodobacteraceae
as a separate deep branch, with 96.8 % or lower sequence similarity values to representatives of the genera
Marivita
,
Donghicola
,
Sulfitobacter
,
Marinovum
,
Phaeobacter
,
Primorskyibacter
,
Roseovarius
and
Aestuariihabitans
. Strain CAU 1492T was closely related to
Marivita geojedonensis
DPG-138T (96.8 %),
Donghicola eburneus
SW-277T (96.7 %),
Sulfitobacter porphyrae
SCM-1T (96.7 %),
Marinovum algicola
FF3T (96.6 %) and
Aestuariihabitans beolgyonensis
BB-MW15T (96.4 %) based on 16S rRNA gene sequences. The major cellular fatty acids of strain CAU 1492T were cyclo-C19 : 0
ω8c and summed feature 8 (C18 : 1
ω7c/C18 : 1
ω6c). The polar lipid pattern was composed of phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified aminolipid. The strain contained Q-10 as the sole respiratory quinone. The draft genome of strain CAU 1492T was 4.63 Mb with a DNA G+C content of 63.1 mol%. The genome includes 4292 protein-coding genes and a five rRNA operons. On the basis of the phenotypic, chemotaxonomic and genomic data, strain CAU 1492T represents a novel genus in the family
Rhodobacteraceae
for which the name Arenibacterium halophilum gen. nov., sp. nov. is proposed. The type strain of Arenibacterium halophilum is CAU 1492T (=KCTC 62998T=NBRC 113696T).
Funder
National Institute of Biological Resources
Chung-Ang University
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
4 articles.
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