Sneathiella marina sp. nov., isolated from a sea anemone in the Western Pacific Ocean

Abstract

A novel marine bacterium designated strain PHK-P5T was isolated from a sea anemone (Actinostolidae sp. 1). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain PHK-P5T belonged to the genus Sneathiella . The bacterium was Gram-stain-negative, aerobic, oxidase- and catalase- positive, oval- to rod-shaped, and motile. Growth was observed at pH 6.0–9.0, salinities of 2.0–9.0 % and temperatures of 4–37 °C. The G+C content of the chromosomal DNA was 49.2 %. The respiratory quinone was determined to be Q-10. The principal fatty acids of strain PHK-P5T were C19 : 0cyclo ω8c (25.19 %), C16 : 0 (22.76 %), summed feature 8 (C18 : 1  ω7c/ω6c; 16.14 %), C14 : 0 (8.81 %), C17 : 0cyclo (8.10 %), summed feature 2 (C12 : 0 aldehyde and/or unknown 10.928; 7.19 %) and C18 : 1  ω7c 11-methyl (5.03 %). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The average nucleotide identity and digital DNA–DNA hybridization values among the genomes of strain PHK-P5T and the reference strains were 68.7–70.9 % and 17.4–18.1 %, respectively. The combined genotypic and phenotypic data showed that strain PHK-P5T represents a novel species within the genus Sneathiella , for which the name Sneathiella marina sp. nov. is proposed, with the type strain PHK-P5T (=MCCC M21824T=KCTC 82924T).