Affiliation:
1. School of Microbiology, University College Cork, Cork, Ireland
2. Teagasc Food Research Centre, Fermoy, Ireland
3. APC Microbiome Ireland, Cork, Ireland
4. Department of Biochemistry & Microbiology, Oklahoma State University – Center for Health Sciences, Tulsa, Oklahoma, USA
Abstract
A novel bacterial strain, APC 3343T, was isolated from the intestine of a deep-sea loosejaw dragon fish, Malacosteus niger, caught at a depth of 1000 m in the Northwest Atlantic Ocean. Cells were aerobic, rod-shaped, yellow/orange-pigmented, non-motile and Gram-negative. Growth of strain APC 3343T was observed at 4–30 °C (optimum, 21–25 °C), pH 5.5–10 (optimum, pH 7–8) and 0.5–8 % (w/v) NaCl (optimum, 2–4 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain APC 3343T was most closely related to members of the genus
Winogradskyella
, with the most closely related type strains being
Winogradskyella algae
Kr9-9T (98.46 % identity),
Winogradskyella damuponensis
F081-2T (98.07 %),
Winogradskyella eximia
CECT 7946T (97.93 %),
Winogradskyella litoriviva
KMM 6491T (97.79 %) and
Winogradskyella endarachnes
HL2-2T (97.79 %). Major fatty acids (>10 % of total) were iso-C16 : 0 3-OH, iso-C15 : 0, anteiso-C15 : 0 and iso-C17 : 0 3-OH. The predominant respiratory quinone was menaquinone-6 (MK-6). Polar lipids were phosphatidylethanolamine, three unknown aminolipids and eight unknown lipids. The draft genome sequence was 3.8 Mb in length with a G+C content of 33.43 mol%. Based on the phenotypic characteristics and phylogenetic analysis, strain APC 3343T is deemed to be a novel species of the genus
Winogradskyella
, and for which the name Winogradskyella bathintestinalis sp. nov. is proposed. The type strain of this species is APC 3343T (=DSM 115832T=NCIMB 15464T).
Funder
Science Foundation Ireland
European Research Council
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
1 articles.
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1. Winogradskyella pelagia sp. nov., isolated from coastal sediment;International Journal of Systematic and Evolutionary Microbiology;2024-07-24