Paramesonia marina gen. nov., sp. nov., isolated from deep-sea water of the Indian Ocean

Abstract

A taxonomic study was carried out of strain K7T, which was isolated from deep-sea water collected from the Indian Ocean. The bacterium was Gram-stain-negative, aerobic, oxidase-negative, catalase-positive, rod-shaped and non-motile. Growth was observed at salinities of 0.5–10 % (optimum, 3 %), at a pH range of pH 6.0–10.0 (optimum, pH 7.0) and at temperatures of 10–40 °C (optimum, 28 °C). Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that strain K7T belonged to the family Flavobacteriaceae , with the high sequence similarities to the genera Mesonia (92.2 %–94.4 %), Salinimicrobium (91.9 %–93.2 %), Salegentibacter (92.1 %–92.6 %), Leeuwenhoekiella (92.1 %–92.3 %), Gramella (91.9 %–92.1 %) and Zunongwangia (91.8 %–92.1 %). The principal fatty acids were iso-C15 : 0 (28.4 %), iso-C15 : 1G (14.2 %), summed feature 9 (iso-C17 : 1  ω9c and/or C16 : 0 10-methyl; 11.6 %), iso-C17 : 0 3-OH (10.0 %) and summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c; 9.6 %). The G+C content of the chromosomal DNA was 35.8 mol%. The respiratory quinone was determined to be MK-6 (100 %). Phosphatidylethanolamine, two unidentified aminolipids, two unidentified phospholipid and four unidentified lipids were detected. The combined genotypic and phenotypic data show that strain K7T represents a novel species of a novel genus, for which the name Paramesonia marina gen. nov., sp. nov. is proposed, with the type strain K7T (=MCCC 1A01093T=KCTC 52325T).