Affiliation:
1. Laboratory of Marine Biology and Ecology, Third Institute of Oceanography, Ministry of Natural Resources, 178 Daxue Road, Xiamen 361005, PR China
2. College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China
Abstract
A Gram–straining–negative, facultatively anaerobic, motile by means of a polar flagellum and rod-shaped marine bacterium, designated S4M6T, was isolated from surface seawater collected in Dongshan Bay (Fujian, PR China). Phylogenetic analysis based on 16S rRNA genes, phylogenomic analysis of single-copy gene families and whole genome data indicated that S4M6T represented a member of the genus
Vibrio
. The closest phylogenetic relatives of S4M6T were
Vibrio marisflavi
CGMCC 1.8994T (97.8 % 16S rRNA gene sequence pairwise similarity),
Vibrio variabilis
LMG 25438T (96.9 %),
Vibrio gangliei
SZDIS-1T (96.2 %) and
Vibrio aestivus
M22T (96.1 %). The growth of S4M6T occurred at 15–35 °C (optimum 28 °C), pH 4.0–9.0 (optimum 5.0–7.0) and in the presence of 2–5 % (w/v) NaCl (optimum 3 %). The predominant fatty acids (>10 %) are C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 :
1ω6c) and summed feature 8 (C18 : 1ω7c and/or C18 :
1ω6c). The DNA G+C content of the assembled genomic sequences was 43.4 % for S4M6T. Average nucleotide identity (ANI) values between S4M6T and the reference species were lower than the threshold for species delineation (95–96 %); in silico DNA–DNA hybridization further indicated that S4M6T had less than 70 % similarity to its relatives. On the basis of the polyphasic evidence, strain S4M6T is proposed to represent a novel species of the genus
Vibrio
, for which the name Vibrio sinus sp. nov. is proposed. The type strain is S4M6T (= KCTC 92312T= MCCC 1K06167T).
Funder
National Natural Science Foundation of China
Marine S&T Fund of Fujian Province
Key Technologies Research and Development Program
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology