Pacificoceanicola onchidii gen. nov., sp. nov., isolated from a marine invertebrate from the South China Sea

Abstract

A Gram-stain-negative, facultative anaerobic, non-flagellated and oval-shaped (0.77–0.98 µm wide and 0.74–1.21 µm long) bacterial strain, designated XY-301T, was isolated from a marine invertebrate collected from the South China Sea. Strain XY-301T grew at 15–37 °C (optimum, 30–35 °C) and at pH 7.0–8.5 (optimum, pH 8.0). The strain was slightly halophilic and it only grew in the presence of 0.5–6.5 % (w/v) NaCl (optimum, 2.5–3.5 %). Its predominant fatty acid (>10 %) was C18 : 1  ω7c. The predominant polar lipids of XY-301T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, six unidentified aminolipids, three unidentified phospholipids and two unknown polar lipids. The respiratory quinone was Q-10. The genome of XY-301T was 4 979 779 bp in size, with a DNA G+C content of 61.3 mol%. The average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values between XY-301T and Pseudoprimorskyibacter insulae SSK3-2T were 73.3, 14.5 and 53.5 %, respectively. Based on the results of phylogenetic, phenotypic, chemotaxonomic and genomic analyses, strain XY-301T is considered to represent a novel species and a new genus of the family Roseobacteraceae , for which the name Pacificoceanicola onchidii gen. nov., sp. nov. is proposed. The type strain is XY-301T (=KCTC 72212T=MCCC 1K03614T).