Citrobacter enshiensis sp. nov., isolated from seleniferous soil

Author:

Zhang Sasa12,Li Kui12,Peng Jing12,Wang Haoyang12,Sun Jiayin12,Guo Yanbin21ORCID

Affiliation:

1. Beijing Key Laboratory of Biodiversity and Organic Farming, China Agricultural University, Beijing, 100193, PR China

2. College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, PR China

Abstract

Two Gram-stain-negative, facultative anaerobic, rod-shaped bacterial strains, S171T and S2-9, were isolated from seleniferous soil in China. Comprehensive phylogenetic analyses based on 16S rRNA genes, multilocus sequences and whole genome sequences indicated that the two strains belonged to the genus Citrobacter . The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values of strains S171T and S2-9 with the closest relative Citrobacter koseri NCTC 10786T were 83.6–83.7% and 27.7–27.8 %, respectively, which were below the species cutoff values. The ANI and dDDH values between the two strains were 97.9% and 84.8 %, respectively. The biochemical characteristics revealed that selenite tolerance, H2S and indole production, arginine dihydrolase, ornithine decarboxylase, as well as acid production from carbon sources such as d-sorbitol and arbutin are distinctive features of the two strains. Based on these results, strain S171T and strain S2-9 represent a novel species of the genus Citrobacter , for which the name Citrobacter enshiensis sp. nov. is proposed, with strain S171T (=GDMCC 1.3637T=JCM 35851T) as the type strain. The genome size of strain S171T was 4.92 Mb with a G+C content of 52.6 mol%. The genome size of strain S2-9 was 4.89 Mb with a G+C content of 52.6 mol%.

Funder

Special Fund for Agro-scientific Research in the Public Interest

Special Fund for Key Science & Technology Program in Xinjiang Province

Special Fund for Key Science & Technology Program in Yunnan Province

Publisher

Microbiology Society

Subject

General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology

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