Optimizing Shelf-life of Pseudomonas fluorescens after Freeze Drying

Abstract

The excess use of chemical fertilizers diminishes soil fertility and yield from crops gradually. To regain and enhance our soil nutrients to get more yields, it is mandatory to rely on soil microbes. Some beneficial microbes’ termed as bio-fertilizers especially rhizosphere bacteria have well contribution in increasing plant growth, and yield without any toxins. It is a very natural process of interaction between plant and some microbes to increase the assimilation of nutrients and it helps plants to enhance better production. In this research, we showed the use of microbes especially Pseudomonas fluorescens survival in the soil. We applied different carriers such as dextrose, talc, and peat with freeze-dried P. fluorescens and studied the shelf-life of the P. fluorescens. Among different carrier’s peat with centrifuged cell suspension survived up to 60 days with significant CFU’s 2×107/gm CFU’s, our research will be a prospective to make new formulations and to increase the shelf-life and survival rate of soil microbes.