Obtaining Cell Cultures of Medicinal Plants

Abstract

In vitro propagation of medicinal plants has been incorporated into producing healthy plants that are beneficial to humanity. Some basic principles and factors tend to influence the cultivation process, thus, causing this method of plant propagation to be adapted owing to the importance and benefits surrounding this method. The main objective of this research work was to obtain cell cultures of medicinal plants of Cichorium intybus, Stevia rebaudiana Bertoni, Monarda citriodora, and Rhodiola krylovii. In obtaining the cell cultures of these medicinal plants, some steps need to be followed. In this research, the effect of different methods of sterilisation/cultivation of plant seeds and explants were evaluated using two different media compositions, observable differences between sterile and non-sterile plant seedlings of C. intybus, Monarda citriodora, and Rhodiola krylovii. The effect of growth regulator (Kinetin) and non-growth regulator (Kinetin) on the cell cultures was observed in solid and liquid media; the dry and wet weight was determined for a callus of Chicory grown in cell suspension culture. All results were presented on tables and charts.