Perspective Chapter: High-Performance Liquid Chromatography Coupled to Mass Spectrometry – The Advance in Chemical Analysis

Abstract

How these techniques coupled, high-performance liquid chromatography and mass spectrometry (LC-MS), revolutionized the field of chemical analysis. For a long time, the field of instrumental chemical analysis was limited, as we could not reliably quantify low-concentration substances in complex matrices. This is certainly one of the main advantages of coupling these techniques. In this perspective chapter, the advantages and limitations of the LC-MS system will be presented, sample preparation for this type of analysis and examples of applications that have had analytical success. It will also be mentioned the analytical advance in relation to miniaturization for lab-in-syringe, lab-on-valve, lab-on-a-chip, organ-on-a-chip, among others.