Allele Mining and Development of Kompetitive Allele Specific PCR (KASP) Marker in Plant Breeding

Abstract

Crop improvement refers to the systematic approach of discovering and selecting plants that possess advantageous alleles for specific target genes. The foundation of crop improvement initiatives typically relies on the fundamental concepts of genetic diversity and the genetic architecture of agricultural plants. Allele mining is a contemporary and efficacious technique utilized for the identification of naturally occurring allelic variations within genes that exhibit advantageous characteristics. Consequently, the utilization of allele mining has significant potential as a feasible approach for enhancing crop-related endeavors. The gene pool of a plant exhibits a substantial degree of genetic variety, characterized by the presence of a multitude of mechanism genes. The utilization of genetic variants for the detection and separation of novel alleles of genes that display favorable traits from the current gene pool, and their subsequent incorporation into the development of improved cultivars through the application of marker-assisted selection, is of utmost importance.