Techniques of Using Peripheral Blood Mononuclear Cells as the Cellular System to Investigate How of the Bovine Species (Indian Zebu-Jersey Crossbreds) Responds to in vitro Thermal Stress Stimulation (Thermal Assault/Heat Shock)

Author:

Olaitan Onasanya Gbolabo,Kannan Thiruvenkadan Aranganoor,Adishetu Yisa Alice,Gopalan Tirumurugaan Krishnaswamy,Nagarajan Murali,Ramasamy Saravanan,Angamuthu Raja,Mutani Msalya George,Obiora Ikeobi Christian

Abstract

Animal production is negatively impacted by global warming and is subject to serious consequences for livestock production systems. In order to understand how PBMCs of Indian Zebu-Jersey crossbreds respond to various levels and durations of thermal assault and heat shock, in this chapter we will discuss techniques involving in vitro thermal stress stimulation (TSS) to stimulate bovine peripheral blood mononuclear cells (PBMCs) under various thermal assault conditions (TACs), including normal to extreme temperatures and varying durations of thermal exposure (DTEs). The consequences of thermal stress on bovine species can be lessened and managed with an understanding of how PBMCs as a cellular system respond to in vitro heat shock and thermal assault. To learn more about how Indian Zebu-Jersey crossbreds respond to in vitro thermal conditions, it may also be possible to explore the relationship between the decrease in PBMCs count during in vitro TSS and the expression of the heat shock protein genes (HSPs) such as HSPs 70 and 90 genes. This will be exploited to discover how Indian Zebu-Jersey crossbreds respond in vivo to diverse environmental thermal conditions and will further enable in vivo understanding of the potential for thermotolerance in bovine species for better adaptability, survival, and production performance.

Publisher

IntechOpen

Reference11 articles.

1. Onasanya GO, Msalya GM, Thiruvenkadan AK, Murali N, Saravanan R, Raja A, et al. Exposure to high thermal conditions for a long time induces apoptosis and decreases total RNA concentration in peripheral blood mononuclear cells among Indian Zebu–Jersey crossbreds. Veterinary World. 2022;15(9):2192-2201

2. Onasanya GO, Msalya GM, Thiruvenkadan AK, Sreekumar C, Tirumurugaan GK, Sanni TM, et al. Single nucleotide polymorphisms at heat shock protein 90 gene and their association with thermo-tolerance potential in selected indigenous Nigerian cattle. Tropical Animal Health Production. 2020;52(4):1961-1970

3. Onasanya GO, Ikeobi CO, Ayotunde AS, Oke FO, Olorunisola RA, Decampos JS. Thermo-regulatory functions of heat shock protein genes in some selected tropically stressed livestock animals. International Journal of Applied Research and Technology. 2017;6(8):37-43

4. Habeeb AAM, Gad AE, Tarabany AA, MAA A. Negative effects of heat stress on growth and milk production of farm animals. Journal of Animal Husbandry Dairy Science. 2018;2(1):1-12

5. Kishore V, Sodhi M, Sharma A, Shandilya UK, Mohanty A, Verma P, et al. Transcriptional stability of heat shock protein genes and cell proliferation rate provides evidence of superior cellular tolerance of Sahiwal (Bos indicus) cow PBMCs to summer stress. Review Journal of Veterinary Science. 2016;2(1):34-40

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