ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation-Reference-Cited by-同舟云学术

ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation

Published:2015-01-01 Issue: Volume:2 Page:10
ISSN:1849-5435
Container-title:Nanobiomedicine
language:en
Short-container-title:Nanobiomedicine

Author:

Smerkova Kristyna¹²,Vlcnovska Marcela¹,Dostalova Simona¹²,Milosavljevic Vedran¹²,Kopel Pavel¹²,Vaculovic Tomas³,Krizkova Sona¹²,Vaculovicova Marketa¹²,Adam Vojtech¹²,Kizek Rene¹²

Affiliation:

1. Department of Chemistry and Biochemistry, Laboratory of Metallomics and Nanotechnology, Mendel University in Brno, Brno, Czech Republic

2. Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic

3. Department of Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic

Abstract

Cisplatin belongs to the most widely used cytostatic drugs. The determination of the presence of the DNA-cisplatin adducts may not only signal the guanine-rich regions but also monitor the interaction reaction between DNA and the drug in terms of speed of interaction. In this work, the combined advantages of magnetic particles-based isolation/purification with fluorescent properties of quantum dots (QDs) and antibodies targeted on specific recognition of DNA-cisplatin adducts are demonstrated. The formation of a complex between magnetic particles with surface modified by anti-dsDNA antibody, cisplatin-modified DNA and QDs labelled anti-cisplatin-modified DNA antibody was suggested and optimized.

Publisher

SAGE Publications

Subject

Biomedical Engineering,Biotechnology

Link

http://journals.sagepub.com/doi/pdf/10.5772/61699

Reference36 articles.

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