THE INCREASED KINETICS OF H2O2-INDUCED CHEMILUMINESCENCE IN THE PATIENTS WITH LONG-TERM CONSEQUENCES AFTER CEREBRAL CONTUSION

Abstract

Abstract Purpose of the study. The aim was to study in the patients with long-term consequences after cerebral contusion the intensity of spontaneous and H2O2-induced chemiluminescence in order to evaluate the early fast-flowing reactions caused by oxidative stress and associated with the formation of primary radicals such as free radical oxidations. Materials and Methods. Forty-two patients with long-term consequences after cerebral contusion were investigated (39,04 ± 12,84 years mean age; mean onset years 32,56 ± 6,4) where both spontaneous and H2O2-induced chemiluminescences were measured directly by HPLC-chemiluminescence assay. Results. The study have showed that sera of the investigated patients with long-term consequences after cerebral contusion have the increased H2O2-induced chemiluminescence associated with the high amplitude of «fast» burst and the tendency to increase of spontaneous chemiluminescence (p = 0,039 and p = 0,58, accordingly). Thus, the patients with long-term consequences after cerebral contusion showed the abnormal high kinetics of H2O2-induced chemiluminescence (p < 0,05). The statistically significant increase serum Н2О2-induced chemiluminescence intensity detected in examined patients (3085,6 ± 114,2 vs 669,1 ± 214,83 controls) have showed the development of certain oxidative stress processes in this category of patients associated with the increasing of primary free radical reactions and their activity were getting increased with the progression of the disease duration (p < 0,05). Conclusions. The study provides the novel data revealing the increased kinetics of H2O2-induced chemiluminescence in the patients with long-term consequences after cerebral contusion accompanied by the tendency to increase of spontaneous chemiluminescence that may play the certain pathogenetic role. Keywords: H2O2-induced chemiluminescence, long-term consequences after cerebral contusion, oxidative stress.