Changes in photosynthetic pigment composition and absorbed energy allocation during salt stress and CAM induction in Mesembryanthemum crystallinum

Abstract

Mesembryanthemum crystallinum L. undergoes a transition from the C3 photosynthetic pathway to crassulacean acid metabolism (CAM) in response to increasing salinity. As a consequence, growth is greatly reduced and less light energy is utilised in carbon fixation, leading to an increase in dissipation of thermal energy to remove potentially dangerous excess excitation energy. The pigment composition of plants grown for 4 weeks at 20 mm (low) and 400 mm (high) NaCl was sampled, and photochemical performance, tissue acidity and growth were sampled at 2 and 4 weeks. High-salt-grown plants, which switched to CAM, accumulated only 25% of the fresh weight of low-salt-grown plants, which maintained C3 photosynthesis. Predawn Fv / Fm and de-epoxidation of violaxanthin [(A + Z) / (V + A + Z)] was similar between plants after 2 and 4 weeks, revealing no sustained depression in PSII efficiency under the high-salt treatment. However, at midday under high photosynthetic photon flux densities (PPFD) high-salt plants displayed lower PSII efficiency, higher (A + Z) / (V + A + Z) and greater allocation of energy to thermal dissipation over photochemistry than low-salt plants. Pigment contents were similar between treatments for the first 3 weeks, but after 4 weeks high-salt plants had accumulated significantly less chlorophyll and lutein than low-salt plants. However, V + A + Z content did not differ. High-salt treatment, leading to CAM photosynthesis and substantial reduction in growth, was associated with increased allocation of energy to xanthophyll cycle-dependent energy dissipation at high light and adjustment of thylakoid pigment composition.