A New Fluorometric Method for the Determination of Oxaliplatin Based on Cucurbit[7]uril Supramolecular Interaction

Abstract

This paper proposed new competitive methods for fluorescence detection of the anti-cancer drug oxaliplatin. The methods were based on the competitive reaction of palmatine (PAL)/berberine (BER)/coptisine (COP) with oxaliplatin for the occupancy of cucurbit[7]uril (CB[7]) cavities. The results showed that the fluorescence intensity of PAL, BER, and COP regularly increased upon addition of CB[7] until a certain amount of oxaliplatin was added, at which stage the fluorescence intensity of the system quenched. Using the CB[7]–PAL, CB[7]–BER, and CB[7]–COP systems, linear ranges in the detection of oxaliplatin of 0.005–1.75, 0.010–1.50, and 0.020–1.05 μg mL–1, with detection limits of 2, 3, and 7 ng mL–1, respectively, were obtained. These results suggest that cucurbit[7]uril is a promising drug carrier for delivering and monitoring targeted oxaliplatin, with improved anti-tumour efficacy and reduced toxicity in normal tissues.