The Application of Enzymic Hydrolysis and Tritium Labelling to a Study of the Modification of Tryptophyl Residues in Proteins

Abstract

The tryptophyl residues of apomyoglobin, S-carboxymethyl-Iysozyme, and wool have been radioactively labelled in order to follow their reactions more easily. Whereas enzymic hydrolysis of these proteins gives good yields of tryptophan, it was found that enzymic hydrolysis of apomyoglobin, after either ozonolysis or treatment with o-nitrophenylsulphenyl chloride, released only part of the resultant modified tryptophyl residues.