Fluorogenic Quantification of Albumin

Abstract

By optimising various fluorogenic dyes, non-fluorescent fluorescamine can react with primary amines to form highly fluorescent products, which is a simple, fast, and sensitive method for the quantification of albumin. The effects of pH, temperature, and chemicals were studied systematically to quantify albumin. The quantification method is more sensitive at alkaline pHs, affording measurement of proteins concentrations as low as 15 µg mL–1. Denaturation of albumin at elevated temperatures and/or use of chemicals, such as ethanol and acetone, can greatly improve the sensitivity of the albumin detection method. The simple, accurate, and reliable analysis of albumin contents under favourable conditions can be developed as an important method for early diagnosis of kidney disease.