Author:
Zhang Bing Rong,Buhr Mary,Kroetsch Tom,Leibo S. P.
Abstract
Fresh spermatozoa from bulls established as ‘good freezers’ and
‘poor freezers’ (consistently ≥50% or
<20% motile spermatozoa after cryopreservation, respectively)
were incubated for 96 h in Tes/Tris–egg yolk or TALP–egg yolk
media at 37°, 20°, 5° or 0°C. The TALP
extender contained 0, 100 or 200 mM glycine betaine (GB) to test the
hypothesis that GB would efficiently maintain spermatozoa function during
long-term incubation. The percentage of motile spermatozoa declined over time
in a temperature- and medium-dependent fashion. No spermatozoa were motile by
24 h incubation at 37°C or by 72 h incubation at 0°C, and
there were no significant differences in the percentage of motile spermatozoa
from either category of bull when spermatozoa were incubated in any media for
less than 24 h. Spermatozoa from poor freezers were significantly more motile
than spermatozoa from good freezers after 96 h at 20° or 5°C
in TALP alone; however, GB at both 100 and 200 mM increased the percentage of
motile spermatozoa in poor and good freezers and eliminated these differences.
Overall, the presence of GB at either 100 or 200 mM significantly improved the
percentage of motile spermatozoa at 20°, 5° and 0°C,
but not at 37°C.
Subject
Developmental Biology,Endocrinology,Genetics,Molecular Biology,Animal Science and Zoology,Reproductive Medicine,Biotechnology
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献