Production of lambs of predetermined sex after the insemination of ewes with low numbers of frozen - thawed sorted X- or Y-chromosome-bearing spermatozoa
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Published:2002
Issue:8
Volume:14
Page:503
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ISSN:1031-3613
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Container-title:Reproduction, Fertility and Development
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language:en
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Short-container-title:Reprod. Fertil. Dev.
Author:
Hollinshead F. K.,O'Brien J. K.,Maxwell W. M. C.,Evans G.
Abstract
The fertilizing ability of sex-sorted frozen–thawed ram spermatozoa was assessed after insemination of mature Merino ewes at a synchronized oestrus. Ewes were inseminated into the uterus or utero–tubal junction (UTJ) with a total of 140 × 106 unsorted (control) or 2–4 × 106 sorted (X or Y) frozen–thawed ram spermatozoa 54�to 57 hours after removal of progestagen-impregnated pessaries and an injection of 400 IU of pregnant mare serum gonadotrophin (Folligon®, Intervet). The spermatozoa were separated into X- and Y-chromosome-bearing spermatozoa after analysis with a modified high-speed cell sorter (SX MoFlo®). The number of ewes pregnant after insemination with unsorted frozen–thawed spermatozoa was significantly higher (26/48; 54.3%) than for ewes inseminated with either X- (12/48; 25.0%) or Y-sorted spermatozoa (7/48; 14.6%) (P<0.05). Seventeen of the eighteen lambs produced by ewes inseminated with X-sorted spermatozoa were female (94.4%) and 8/8 lambs from ewes inseminated with Y-sorted spermatozoa were male (100%). The sex ratio of the lambs born to ewes inseminated with sex-sorted spermatozoa was significantly skewed from the 51.3% male and 48.7% female ratio in the control group (P<0.05). This study showed, for the first time, that lambs of predicted sex can be produced after insemination with low numbers of sex-sorted cryopreserved ram spermatozoa.
Publisher
CSIRO Publishing
Subject
Developmental Biology,Endocrinology,Genetics,Molecular Biology,Animal Science and Zoology,Reproductive Medicine,Biotechnology
Cited by
58 articles.
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