Effect of elevated temperatures on bovine corpus luteum function: expression of heat-shock protein 70, cell viability and production of progesterone and prostaglandins by cultured luteal cells

Abstract

Summer heat stress lowers fertility in cattle in hot environments by influencing oocyte quality, follicular activity and progesterone (P4) level in blood plasma. However, the mechanisms by which elevated temperature influences corpus luteum function remain unclear. Elevated temperature has generally been known to upregulate the gene expression of heat-shock protein (HSP) 70 in a variety of cell types. To clarify the direct effects of elevated temperature on bovine corpus luteum function, we examined the expressions of HSP70, cell viability and the production of P4 and prostaglandins (PGs) in luteal cells cultured at 37.5°C (normal temperature in our culture system), 39.0°C (moderately elevated temperature) or 41.0°C (severely elevated temperature) for 12 or 24 h. HSP70 mRNA expression was increased by incubation at 39.0°C for 12 h and at 41.0°C for 12 and 24 h, whereas HSP70 protein expression was not significantly affected. The viability of luteal cells cultured for 24 h, measured by flow cytometry with propidium iodide staining, was not significantly affected by temperature. Interestingly, the production of P4 by cultured luteal cells was higher at 39.0°C than at 37.5°C after 12 and 24 h of incubation. The production of PGF2α was higher at 39.0°C and 41.0°C than at 37.5°C after 12 and 24 h of incubation. The production of PGE2 was higher at 41.0°C than at 37.5°C after 24 h of incubation. The overall results suggested that elevated temperature does not negatively affect luteal function, and that the low fertility observed during summer is not due to a direct effect of elevated temperature on luteal cells.