Author:
Cha KY,Do BR,Chi HJ,Yoon TK,Choi DH,Koo JJ,Ko JJ
Abstract
Immature human follicular oocytes were collected from unstimulated ovaries, matured and fertilized
in vitro and then transferred to patients with no ovarian dysfunction such as premature ovarian failure.
From 11 1 consenting donors, 422 immature oocytes were collected from 97 ovaries between January
1990 and October 1991. The number of oocytes collected from ovaries and their development were
recorded so that comparisons could be made among donors of different ages and ovarian condition,
such as menstrual cycle, cyclic and non-cyclic ovaries. The rate of fertilization in vitro showed a peak
in the 31-40-year age group; however, there was no statistical difference in the rate of oocyte maturation
and cleavage among the donors in the different age groups. Immature oocytes of the luted phase had
a significantly higher maturation rate than those of the follicular phase. There was no significant
difference in the number of recovered oocytes, or in the development of immature follicular oocytes,
between cyclic and non-cyclic ovaries. Mature follicular fluid and peritoneal fluid had a significant
effect on the development of immature follicular oocytes. Also, it was found that fertilized eggs
cleaved more frequently in the medium containing hypoxanthine compared with the medium without
hypoxanthine. Finally, from 21 transfer cycles, viable embryos were derived from immature follicular
oocytes, resulting in two pregnancies, both leading to the birth of normal babies. These findings
suggest that culture in vitro of immature follicular oocytes, from unstimulated ovaries, to a suitable
condition, could be used optimally for clinical applications such as human ovum donation
programmes.
Subject
Developmental Biology,Endocrinology,Genetics,Molecular Biology,Animal Science and Zoology,Reproductive Medicine,Biotechnology
Cited by
35 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献